Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. Western blot analysis of various lysates, using ASGR1 antibody (A6871) at 1:500 dilution. The second-step reagent was PE Goat Anti-Mouse Ig (Cat. 563654 solid line histogram) at matched concentrations. 554121 dashed line histogram) or Purified Mouse Anti-ASGPR 1 antibody (Cat. The protein encoded by this gene is the more. The cells were stained with either Purified Mouse IgG1, Isotype Control (Cat. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. Simplified quantification method for in vivo SPECT/CT imaging of asialoglycoprotein receptor with (99m)Tc-p(VLA-co-VNI) to assess and stage hepatic fibrosis in mice. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Systemically Administered Plant Recombinant Holo-Intrinsic Factor Targets the Liver and is not Affected by Endogenous B12 levels. Anti-ASGR1 Antibody (APC), Mouse Monoclonal Validated applications. The monoclonal antibody 8D7 is cross reactive with human ASGPR. The monoclonal antibody 8D7 recognizes a subunit-specific epitope of RHL-1 of rat ASGPR. Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers. The rat asialoglycoprotein receptor (ASGPR) consists of three polypeptide subunits: rat hepatic lectin (RHL)-1, RHL-2 and RHL-3. Publishing research using ab88042? Please let us know so that we can cite the reference in this datasheet.Īb88042 has been referenced in 5 publications. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406 Immunohistochemistry analysis of paraffin-embedded mouse liver using ASGR1 antibody (A16766) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol. The membrane was then blocked for an hour using 1% Milk/TBST before being incubated with ab88042 overnight at 4☌.
The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. We are unsure as to the identity of these extra bands. Observed band size: 44,48 kDa why is the actual band size different from the predicted?Īdditional bands at: 140 kDa.
All lanes : Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042) at 1 µg/mlĪll lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
0 kommentar(er)
